REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY OF PHOSPHOLIPASES: APPLICATION OF SPECTROPHOTOMETRIC DETECTION TO RAT PHOSPHOLIPASE A2 ISOZYMES

This paper appraises an HPLC method for assaying phospholipase A2 (PLA2).The procedure is based on heptane-isopropanol-H2SO4 extraction of fatty acids released by the enzyme in the presence of margaric acid as an internal standard, and precolumn derivatization with 9-anthryldiazomethane.The derivatives of naturally occurring long-chain fatty acids

Knowledge of wind effects is of great significance in structural, environmental, and architectural fields, where excessive relevance among wind pressure, building load, and natural ventilation has been formerly confirmed.Within the scope of high-rise buildings, functions of their layout, separation and height in altering wind pressure have been inq

The ability of a firm to endure, adapt and prosper cashel tail bag over time is a multifaceted outcome influenced by an interplay of internal and external factors.However, the precise mechanisms and specific dynamics that contribute to sustained firm survival remain unclear.This study investigated the moderating effect of risk management committee

At the awakening of the new 5G network as the network of services, issues related to electromagnetic fields (EMFs) will become one of the key aspects for the cost-effective establishment of the 5G infrastructure.The new 5G services will cashel tail bag meet the rigorous demand for bandwidth through the implementation of a large number of densely lo